Cell-free synthesis of herpes simplex virus proteins

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Publication Type Journal Article
School or College College of Science; College of Science; College of Engineering
Department Bioengineering; Biology; Human Genetics
Creator Gesteland, Raymond F.
Other Author Summers, William C.; Cremer, Kenneth J.
Title Cell-free synthesis of herpes simplex virus proteins
Date 1977
Description Polyribosomes isolated from herpes simplex virus type I (HSV-1)-infected cells have been used to program a eucaryotic cell-free translation system. At least 10 HSV-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type HSV-infected polyribosomes. Polyribosomes prepared from thymidine kinase-negative mutants of HSV direct the synthesis of three putative nonsense termination polypeptides. HSV-specific polypeptides synthesized in vitro are precipitated with antiserum to HSV-infected cell proteins.
Type Text
Publisher American Society for Microbiology
Volume 22
Issue 3
First Page 750
Last Page 757
Subject Viral Proteins; Herpes Simplex; Peptide Biosynthesis; Thymidine Kinase
Subject MESH Cell-Free System; Viral Proteins; Polyribosomes; Molecular Weight
Language eng
Bibliographic Citation Cremer, K.J., W.C. Summers and R.F. Gesteland. 1977. Cell-free synthesis of herpes simplex virus proteins. Journal of Virology, 22(3), 750-7.
Rights Management ©American Society for Microbiology
Format Medium application/pdf
Identifier ir-main,12757
ARK ark:/87278/s6t731qb
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-08-30
ID 703999
Reference URL https://collections.lib.utah.edu/ark:/87278/s6t731qb
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