Targeted mutagenesis by homologous recombination in D. melanogaster

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Publication Type Journal Article
School or College College of Science
Department Biology
Creator Olivera, Baldomero M.
Other Author Rong, Yikang S.; Titen, Simon W.; Xie, Heng B.; Golic, Mary M.; Bastiani, Michael; Bandyopadhyay, Pradip; Brodsky, Michael; Rubin, Gerald M.; Golic, Kent G.
Title Targeted mutagenesis by homologous recombination in D. melanogaster
Date 2002
Description We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate targeted alterations of the host genome. The results address several questions about the general utility of this technique. We show that genes not near telomeres can be efficiently targeted; that no knowledge of the mutant phenotype is needed for targeting; and that insertional mutations and allelic substitutions can be easily produced.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 16
Issue 12
First Page 1568
Last Page 1581
Language eng
Bibliographic Citation Rong, Y. S., Titen, S. W., Xie, H. B., Golic, M. M., Bastiani, M., Bandyopadhyay, P., Olivera, B. M., Brodsky, M., Rubin, G. M., & Golic, K. G. (2002). Targeted mutagenesis by homologous recombination in D. melanogaster. Genes & Development, 16(12), 1568-81.
Rights Management © Cold Spring Harbor Laboratory Press-CSHL Press, © 2002 Cold Spring Harbor Laboratory Press-CSHL Press ; Reprinted from Rong, Y. S., Titen, S., Xie, H., Golic, M. M., Bastiani, M., Bandyopadhyay, P., Olivera, B. M., Brodsky, M., Rubin, G. M., & Golic, K. G. (2002). Targeted mutagenesis by homologous recombination in Drosophila melanogaster. Genes & Development, 16(12), 1568-81.
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Format Extent 550,734 bytes
Identifier ir-main,8415
ARK ark:/87278/s6tt5864
Setname ir_uspace
ID 703879
Reference URL https://collections.lib.utah.edu/ark:/87278/s6tt5864
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