BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae

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Publication Type Journal Article
School or College College of Science
Department Chemistry; Mathematics
Creator Poulter, Charles Dale; Jiang, Yunfeng
Other Author Proteau, P.; Ferro-Novick, S.
Title BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae
Date 1995-09-15
Description Protein prenylation utilizes different types of isoprenoids groups, namely farnesyl and geranylgeranyl, to modify proteins. These lipophilic moieties attach to carboxyl-terminal cysteine residues to promote the association of soluble proteins to membranes. Most prenylated proteins are geranylgeranylated. Geranylgeranylation is catalyzed by two different prenyltransferases, the type I and type II geranylgeranyl transferases, both of which utilize geranylgeranyl diphosphate as a lipid donor. In the yeast Saccharomyces cerevisiae, the BET2 gene encodes the beta-subunit of the type II geranylgeranyl transferase. Mutations in this gene cause a defect in the geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic. In an attempt to analyze those genes whose products may interact with Bet2, we isolated a suppressor of the bet2-1 mutant. This suppressor gene, called BTS1, encodes the yeast geranylgeranyl diphosphate synthase. BTS1 is not essential for the vegetative growth of cells; however, disrupting it impedes the geranylgeranylation of many cellular proteins and renders cells cold sensitive for growth. Our findings imply that BTS1 suppresses the bet2-1 mutant by increasing the intracellular pool of geranylgeranyl diphosphate.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 270
Issue 37
First Page 21793
Last Page 21799
Subject Amino Acid Sequence; Cloning, Molecular; Gene Expression
Subject MESH Alkyl and Aryl Transferases; Oxidoreductases;Saccharomyces cerevisiae
Language eng
Bibliographic Citation Poulter, C. D. (1995). BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae. Journal of Biological Chemistry 270(37), 21793-9
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Format Extent 472,912 bytes
Identifier ir-main,1981
ARK ark:/87278/s6h13k6c
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2014-05-28
ID 703577
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