| Publication Type |
journal article |
| School or College |
College of Science |
| Department |
Biology |
| Creator |
Capecchi, Mario R.; Thomas, Kirk R. |
| Title |
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells |
| Date |
1987 |
| Description |
We mutated, by gene targeting, the endogenous hypoxanthine phosphorlbosyl transferase (HPFlT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (NO') gene was introduced into an exon of a cloned fragment of the Hprf gene and used to transfect ES cells. Among the G418' colonies, l/l000 were also resistant to the base analog &thioguanine (&TG). The G418', 8-TGr cells were all shown to be Hprt- as the result of homologous recombination with the exogenous, neo'-containing, Hprf sequences. |
| Type |
Text |
| Publisher |
MIT Press |
| First Page |
503 |
| Last Page |
512 |
| Subject |
Site-directed mutagenesis; Hypoxanthine phosphorlbosyl transferase gene; HPFlT gene; Neomycin resistance gene; Homologous recombination; Mouse stem cells; Mouse embryo-derived stem cells |
| Subject LCSH |
Gene targeting; Recombinant DNA; Mutagenesis; Mice -- Genetics; Stem cells |
| Language |
eng |
| Bibliographic Citation |
Thomas, K. R., & Capecchi, M. R. (1987). Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell, 51, 503-12. |
| Rights Management |
© MIT Press http://mitpress.mit.edu/ |
| Format Medium |
application/pdf |
| Format Extent |
1,805,150 bytes |
| Identifier |
ir-main,8977 |
| ARK |
ark:/87278/s61c2f2n |
| Setname |
ir_uspace |
| ID |
703513 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s61c2f2n |
