Generating mice with targeted mutations.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Title Generating mice with targeted mutations.
Date 2001-10-01
Description Mutational analysis is one of the most informative approaches available for the study of complex biological processes. It has been particularly successful in the analysis of the biology of bacteria, yeast, the nematode worm Caenorhabditis elegans and the fruit fly Drosophila melanogaster. Extension of this approach to the mouse, through informative, was far less successful relative to what has been achieved with these simpler model organisms. This is because it is not numerically practical in mice to use random mutagenesis to isolate mutations that affect a specified biological process of interest. Nonetheless, biological phenomena such as a sophisticated immune response, cancer, vascular disease or higher-order cognitive function, to mention just a few, must analyzed in organisms that show such phenomena, and for this reason geneticists and other researchers have turned to the mouse. Gene targeting, the means for creating mice with designed mutations in almost any gene, was developed as an alternative to the impractical use of random mutgenesis for pursing genetic analysis in the mouse. Now gene targeting has advanced the genomic manipulations possible in mice to a level that can be matched only in far simple organisms such as bacteria and yeast.
Type Text
Publisher Nature Publishing Group
Journal Title Nature Medicine
Volume 7
Issue 10
First Page 1086
Last Page 1090
DOI 10.1038/nm1001-1086
citatation_issn 10788956
Subject Animals; Recombination, Genetic; Stem Cells
Subject MESH Awards and Prizes; Gene Targeting; Mice, Mutant Strains
Language eng
Bibliographic Citation Nat Med. 2001 Oct;7(10):1086-90. Capecchi MR. Generating mice with targeted mutations. Retrieved on November 1 2006 from
Rights Management copyright © 2001 Nature Publishing Group. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,526
ARK ark:/87278/s6765zhz
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 703260
Reference URL
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