Hox group 3 paralogous genes act synergistically in the formation of somitic and neural crest-derived structures.

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Publication Type Journal Article
School or College School of Medicine; College of Science
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Manley, Nancy R.
Title Hox group 3 paralogous genes act synergistically in the formation of somitic and neural crest-derived structures.
Date 1997-12-15
Description Hox genes encode transcription factors that are used to regionalize the mammalian embryo. Analysis of mice carrying targeted mutations in individual and multiple Hox genes is beginning to reveal a complex network of interactions among these closely related genes which is responsible for directing the formation of spatially restricted tissues and structures. In this report we present an analysis of the genetic interactions between all members of the third paralogous group, Hoxa3, Hoxb3, and Hoxd3. Previous analysis has shown that although mice homozygous for loss-of-function mutations in either Hoxa3 or Hoxd3 have no defects in common, mice mutant for both genes demonstrate that these two genes strongly interact in a dosage-dependent manner. To complete the analysis of this paralogous gene family, mice with a targeted disruption of the Hoxb3 gene were generated. Homozygous mutants have minor defects at low penetrance in the formation of both the cervical vertebrae and the IXth cranial nerve. Analysis and comparison of all double-mutant combinations demonstrate that all three members of this paralogous group interact synergistically to affect the development of both neuronal and mesenchymal neural crest-derived structures, as well as somitic mesoderm-derived structures. Surprisingly, with respect to the formation of the cervical vertebrae, mice doubly mutant for Hoxa3 and Hoxd3 or Hoxb3 and Hoxd3 show an indistinguishable defect, loss of the entire atlas. This suggests that the identity of the specific Hox genes that are functional in a given region may not be as critical as the total number of Hox genes operating in that region.
Type Text
Publisher Academic Press Ltd.
Volume 192
Issue 2
First Page 274
Last Page 288
Subject Abnormalities, Multiple; Gene Targeting; Glossopharyngeal Nerve; Mice, Knockout; Morphogenesis
Subject MESH Cervical Vertebrae; DNA-Binding Proteins; Embryonic and Fetal Development; Gene Expression Regulation, Developmental; Genes, Homeobox; Somites; Xenopus Proteins
Language eng
Bibliographic Citation Dev Biol. 1997 Dec 15;192(2):274-88. Manley NR, Capecchi MR. Hox group 3 paralogous genes act synergistically in the formation of somitic and neural crest-derived structures. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WDG-45SJ7Y7-6&_coverDate=12%2F15%2F1997&_alid=479378117&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6766&_sort=d&view=c&_acct=C000049956&_version=1&_urlVersion=0&_userid=990933&md5=5f65b5a9d9b52365f7cea5af5d284eb2.
Rights Management Copyright ? 1997 Academic Press. All rights reserved.
Format Medium application/pdf
Identifier ir-main,535
ARK ark:/87278/s6zc8m2c
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 702940
Reference URL https://collections.lib.utah.edu/ark:/87278/s6zc8m2c
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