|School or College
|School of Medicine; College of Science; College of Engineering
|Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
|Gesteland, Raymond F.; Ives, Jeffrey T.; Stockham, Thomas G.
|Automated film reader for DNA sequencing based on homomorphic deconvolution
|An automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homomorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base calling. Initial reads from direct blot sequencing films have error rates of about 1% at the rate of 5 nucleotides/s. Typical read lengths are 500-600 nucleotides. The described reader is a significant improvement over existing readers and could be an essential component in the sequencing efforts of the Human Genome Project.
|Institute of Electrical and Electronics Engineers (IEEE)
|Sequence Analysis; Electrophoresis; Automated Film Reader; Homomorphic Deconvolution
|Human Genome Project; Molecular Sequence Data
|Ives, J.T., Gesteland, R.F., and Stockham, T.G. 1994. An automated film reader for DNA sequencing based on homomorphic deconvolution. IEEE Transactions on Biomedical Engineering 41(6): 509-519
|© Institute of Electrical and Electronics Engineers (IEEE)