Automated film reader for DNA sequencing based on homomorphic deconvolution

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Publication Type Journal Article
School or College School of Medicine; College of Science; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Ives, Jeffrey T.; Stockham, Thomas G.
Title Automated film reader for DNA sequencing based on homomorphic deconvolution
Date 1994
Description An automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homomorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base calling. Initial reads from direct blot sequencing films have error rates of about 1% at the rate of 5 nucleotides/s. Typical read lengths are 500-600 nucleotides. The described reader is a significant improvement over existing readers and could be an essential component in the sequencing efforts of the Human Genome Project.
Type Text
Publisher Institute of Electrical and Electronics Engineers (IEEE)
Volume 41
Issue 6
First Page 509
Last Page 519
Subject Sequence Analysis; Electrophoresis; Automated Film Reader; Homomorphic Deconvolution
Subject MESH Human Genome Project; Molecular Sequence Data
Language eng
Bibliographic Citation Ives, J.T., Gesteland, R.F., and Stockham, T.G. 1994. An automated film reader for DNA sequencing based on homomorphic deconvolution. IEEE Transactions on Biomedical Engineering 41(6): 509-519
Rights Management © Institute of Electrical and Electronics Engineers (IEEE)
Format Medium application/pdf
Identifier ir-main,12761
ARK ark:/87278/s6794nsg
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 702670
Reference URL https://collections.lib.utah.edu/ark:/87278/s6794nsg