Modulation of macrophage and microglial responses to axonal injury in the peripheral and central nervous systems

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Publication Type Journal Article
School or College School of Medicine
Department Neurosurgery
Creator Dailey, Andrew T.
Other Author Lazar, Daniel A.; Ellegala, Dilantha B.; Avellino, Anthony M.; Andrus, Kate; Kliot, Michel
Title Modulation of macrophage and microglial responses to axonal injury in the peripheral and central nervous systems
Date 1999
Description OBJECTIVE: After axonal injury, macrophages rapidly infiltrate and become activated in the mammalian peripheral nervous system (PNS) but not the central nervous system (CNS). We used the dorsal root pathway to study factors that modulate the response of macrophages to degenerating axons in both the PNS and the CNS. METHODS: Lewis rats underwent transection of dorsal roots (Group I), stab within the spinal cord (Group II), crush at the dorsal root entry zone (Group III), transection of dorsal roots combined with a CNS lesion (Group IV), or systemic administration of a known activator of macrophages, lipopolysaccharide, alone (Group V) or combined with transection of dorsal roots (Group VI). ED-1 antibody stained for macrophages and activated microglia at 7, 14, and 42 days postinjury. RESULTS: At early time points, Group I demonstrated ED-1 cells in the PNS but not the CNS portion of the degenerating dorsal roots. Group II revealed ED-1 cells near the stab lesion. Group III demonstrated ED-1 cells adjacent to the dorsal root entry zone crush site. Group IV revealed ED-1 cells along both the PNS and the CNS portions of the degenerating dorsal roots when the CNS lesion was placed near the transected roots. Group V demonstrated few ED-1 cells in the PNS and the CNS, whereas Group VI revealed a marked ED-1 cellular response along both the PNS and the CNS portions of the transected dorsal roots. CONCLUSION: Local CNS trauma and systemic administration of lipopolysaccharide can "prime" macrophages/microglia, resulting in an enhanced response to degenerating axons in the CNS. Such priming might prove useful in promoting axonal regeneration.
Type Text
Publisher Wolters Kluwer (LWW)
Volume 45
Issue 3
First Page 593
Last Page 600
Subject Axonal injury; Macrophage response; Microglial response; Peripheral nervous system; Lewis rats
Subject LCSH Axons -- Wounds and injuries; Macrophages -- Activation; Microglia; Nerves, Peripheral; Central nervous system
Language eng
Bibliographic Citation Lazar, D. A., Ellegala, D. B., Avellino, A. M., Dailey, A. T., Andrus, K., & Kliot, M. (1999). Modulation of macrophage and microglial responses to axonal injury in the peripheral and central nervous systems. Neurosurgery, 45(3), 593-600.
Rights Management (c) Wolters Kluwer (LWW) http://lww.com
Format Medium application/pdf
Format Extent 644,984 bytes
Identifier ir-main,13121
ARK ark:/87278/s6qf9b02
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 702643
Reference URL https://collections.lib.utah.edu/ark:/87278/s6qf9b02
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