| Publication Type |
Journal Article |
| School or College |
College of Science |
| Department |
Biology |
| Creator |
Hughes, Kelly T. |
| Other Author |
Chadsey, Meggen S.; Karlinsey, Joyce E. |
| Title |
Flagellar anti-σ factor FlgM actively dissociates Salmonella typhimurium σ28 RNA polymerase holoenzyme |
| Date |
1998 |
| Description |
The anti-σ factor FlgM of Salmonella typhimurium inhibits transcription of class 3 flagellar genes through a direct interaction with the flagellar-specific σ factor, σ28. FlgM is believed to prevent RNA polymerase (RNAP) holoenzyme formation by sequestering free σ28. We have analyzed FlgM-mediated inhibition of σ28 activity in vitro. FlgM is able to inhibit σ28 activity even when σ28 is first allowed to associate with core RNAP. Surface plasmon resonance (SPR) was used to evaluate the interaction between FlgM and both σ28 and σ28 holoenzyme (Eσ28). |
| Type |
Text |
| Publisher |
Cold Spring Harbor Lab Press |
| Volume |
12 |
| Issue |
19 |
| First Page |
3123 |
| Last Page |
3136 |
| Subject |
sigma-factors; transcription; FlgM; Flagellum |
| Subject LCSH |
Salmonella typhimurium; Genetic transcription; RNA polymerases; Flagella (Microbiology) |
| Language |
eng |
| Bibliographic Citation |
Chadsey, M. S., Karlinsey, J. E., & Hughes, K. T. (1998). Flagellar anti-σ factor FlgM actively dissociates Salmonella typhimurium σ28 RNA polymerase holoenzyme. Genes & Development, 12(19), 3123-36. |
| Rights Management |
(c) Cold Spring Harbor Laboratory Press-CSHL Press |
| Format Medium |
application/pdf |
| Format Extent |
585,898 bytes |
| Identifier |
ir-main,7232 |
| ARK |
ark:/87278/s63r1b0j |
| Setname |
ir_uspace |
| ID |
702640 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s63r1b0j |
