Development of a bioabsorbalbe active release antimicrobial coating for orthopedic implants

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Publication Type honors thesis
School or College College of Engineering
Department Bioengineering
Faculty Mentor Dustin L. Williams
Creator Luo, Shirley
Title Development of a bioabsorbalbe active release antimicrobial coating for orthopedic implants
Year graduated 2016
Date 2016-05
Description Orthopedic implantations often create an opportunity for biofilm-related infections to arise. Patients infected with biofilm must often undergo additional surgeries for repair or replacement, which puts them at a greater risk of osteomyelitis, sepsis, and other complications including death [17]. This study investigated the potential of an active release antimicrobial coating to eradicate ethicillin -resistant Staphylococcus aureus (MRSA). MRSA is commonly found in the biofilm phenotype and is potentially fatal. A coating for orthopedic devices would reduce risks of infection and increase success rates. To test the potential of a combination coating of the antimicrobial agent, CZ-1-99, and Poly-co-lactic-glycolicacid (PLGA), a three-phase experimental process was developed. Phase one consisted of determining the minimum inhibitory concentration (MIC) of CZ-1-99 against planktonic bacteria, phase two consisted of determining the effective biofilm eradication concentration (EBEC), and phase three was to incorporate CZ-1-99 into PLGA and determine its initial efficacy against planktonic bacteria of MRSA. The MIC of CZ-1-99 was 5μg/mL. In the EBEC assay, a CZ-1-99 concentration of 400μg/mL was able to reduce biofilm by 6 log 10 units. An addition of 10% CZ-1-99 to the PLGA coating significantly impaired the growth of planktonic MRSA, but additional data will be needed to optimize the coating. The results of the three tests provided an indication that a CZ-1-99 may have promise to eradicate planktonic MRSA and further work will be needed to optimize its efficacy against MRSA in the biofilm phenotype.
Type Text
Publisher University of Utah
Subject Orthopedic implants - Research; Anti-infective agents - Research; Staphyloccoccal infections - Prevention and control - Research; Antimicrobial coating; Staphylococcus aureus
Language eng
Rights Management (c) Shirley Luo
Format Medium application/pdf
Format Extent 25,097 bytes
Identifier honors/id/49
Permissions Reference URL
ARK ark:/87278/s6865rqg
Setname ir_htoa
Date Created 2016-06-28
Date Modified 2018-10-30
ID 205701
Reference URL
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