A novel tool to capture neural activity across an entire brain using C-FOS as an indicator

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Publication Type honors thesis
School or College College of Engineering
Department Biomedical Engineering
Faculty Mentor Dimitri Trankner
Creator Gilbert, John
Title A novel tool to capture neural activity across an entire brain using C-FOS as an indicator
Year graduated 2016
Date 2016-05
Description Immunohistochemical staining for the expression of the immediate early gene c-Fos is a powerful tool to measure neuronal activation of neurons across an entire brain. The results of c-Fos immunostaining are often quantified by counting the number of immunolabeled cells in a region of interest (ROI) determined by the experimental conditions. While powerful for confirming differences in neural activity in the ROI, this technique is time-consuming, inefficient, and ignores valuable information about the widespread network activity across an entire brain. In order to analyze c-Fos expression across an entire brain, we developed a novel Comprehensive Expression and Activity Assessment Tool (CEAT). CEAT is a semi-automated tool to identify cells in brain slices, quantify the activity through a variety of measures, and locate the activity within a particular region in the brain. In this paper we used a simple experiment examining visual processing in a mouse model in order to demonstrate the validity of the tool. Subsequent experiments will use this tool explore differences in neuronal activity of mouse models for neurological disorders in comparison to wild type mice.
Type Text
Publisher University of Utah
Subject Brain - Localization of functions - Research; Brain - Physiology - Research; Brain mapping - Research; Memory - Research; Mice as laboratory animals - Research; Brain activity; Immunohistochemical staining
Language eng
Rights Management (c) John Gilbert
Format Medium application/pdf
Format Extent 25,055 bytes
Identifier honors/id/22
Permissions Reference URL https://collections.lib.utah.edu/details?id=1256319
ARK ark:/87278/s6rc0dv9
Setname ir_htoa
Date Created 2016-06-27
Date Modified 2018-10-30
ID 205674
Reference URL https://collections.lib.utah.edu/ark:/87278/s6rc0dv9
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