The role of HFL1 in lysosomal homeostasis

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Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Lilavivat, Seth
Title The role of HFL1 in lysosomal homeostasis
Date 2013-12
Description In eukaryotes, lysosomes are dynamic organelles that change their size and shape depending on environmental and biological conditions. An emerging paradigm in lysosome biology is that size and copy number are regulated by an equilibrium between continuous cycles of fusion and fission. We discovered a novel gene, SPAC30D11.06c, that encodes a lysosomal membrane protein. The gene was appropriately named HFL1 (Has Fused Lysosomes) because in the null mutant, perinuclear lysosomes fuse together into long tubules. In this work, we established biochemical conditions to solubilize and purify HFL1 from crude cell lysate and performed a co-immuniprecipitation of HFL1 to identify possible protein partners that might inform on HFL1 function. The results of this study demonstrate that HFL1 can be isolated and studied in vitro providing important tools for future studies exploring HFL1 function.
Type Text
Publisher University of Utah
Subject HFL1; Lysosome; Lysosome dynamics; Lysosome fusion; Lysosome homeostasis; Pombe
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Rights Management Copyright © Seth Lilavivat 2013
Format Medium application/pdf
Format Extent 543,159 bytes
Identifier etd3/id/2693
ARK ark:/87278/s6tn0k9k
Setname ir_etd
Date Created 2014-02-07
Date Modified 2021-05-06
ID 196268
Reference URL https://collections.lib.utah.edu/ark:/87278/s6tn0k9k
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