Functional and Morphologic Comparison of Two Methods of Produce Transient Retinal ischemia in the Rat

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Title Journal of Neuro-Ophthalmology, March 2001, Volume 21, Issue 1
Date 2001-03
Language eng
Format application/pdf
Type Text
Publication Type Journal Article
Collection Neuro-Ophthalmology Virtual Education Library: Journal of Neuro-Ophthalmology Archives: https://novel.utah.edu/jno/
Publisher Lippincott, Williams & Wilkins
Holding Institution Spencer S. Eccles Health Sciences Library, University of Utah
Rights Management © North American Neuro-Ophthalmology Society
ARK ark:/87278/s6768mdm
Setname ehsl_novel_jno
ID 225148
Reference URL https://collections.lib.utah.edu/ark:/87278/s6768mdm

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Title Functional and Morphologic Comparison of Two Methods of Produce Transient Retinal ischemia in the Rat
Creator Rosenbaum, DM; Rosenbaum, PS; Singh, M; Gupta, G; Gupta, H; Li, B; Roth, S
Affiliation Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Abstract OBJECTIVES: Much of our knowledge of the pathophysiology of retinal ischemic injury is from a multitude of studies that use in vitro or in vivo animal models of retinal ischemia followed by reperfusion. The objective of this study was to compare histopathologic and electrophysiologic (electroretinography) parameters using two different models of transient retinal ischemia: high intraocular pressure (HIOP) and suture ligation of the optic nerve (SL). METHODS: Transient retinal ischemia was induced using the HIOP model or the SL model in the Sprague-Dawley rat for either 30 or 60 minutes. Histopathologic outcome was determined at 1 and 7 days after ischemia. In addition, electroretinography (ERG) was performed at 2 hours, I day, 3 days, and 7 days after ischemia. RESULTS: At 1 and 7 days after 30 minutes of ischemia, there were no significant histopathologic abnormalities in the retina with either model, except for a slight decrease of the cell count in the ganglion cell layer (GCL) with the SL method. After 60 minutes of ischemia, there was significant thinning of the inner retina. There was a significant early dropout of cells at 1 day in the inner nuclear layer (INL) in the HIOP method compared to the SL method where the dropout was delayed and gradually progressive. Dropout of cells in the GCL was early (I day) and gradually progressive in both models but more severe in HIOP than SL. There was a significant decrease in the ERG b-wave amplitudes as early as 2 hours after both 30 and 60 minutes of ischemia compared to preischemic baselines. CONCLUSIONS: The degree of retinal injury after transient retinal ischemia was more severe at 1 day after reperfusion in the HIOP method compared to the SL method but was similar at 7 days in both models. Furthermore, our data suggests that functional assessment of ischemic damage by electroretinography may be a more sensitive parameter than conventional histopathologic quantification. The timing of either measurement relative to the ischemic stimulus is critical because histologic measurements performed too early after ischemia may underestimate the degree of injury.
Subject Animals; Electroretinography; Intraocular Pressure; Ligation/methods; Models, Animal; Ocular Hypertension/complications; Ophthalmic Artery/physiopathology; Optic Nerve/blood supply; Rats; Rats, Sprague-Dawley; Reperfusion Injury/etiology/pathology/physiopathology; Retina/physiopathology; Retinal Diseases/etiology/pathology/physiopathology
OCR Text Show
Format application/pdf
Publication Type Journal Article
Collection Neuro-Ophthalmology Virtual Education Library: Journal of Neuro-Ophthalmology Archives: https://novel.utah.edu/jno/
Publisher Lippincott, Williams & Wilkins
Holding Institution Spencer S. Eccles Health Sciences Library, University of Utah
Rights Management © North American Neuro-Ophthalmology Society
Setname ehsl_novel_jno
ID 225145
Reference URL https://collections.lib.utah.edu/ark:/87278/s6768mdm/225145
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