Publication Type |
Journal Article |
School or College |
College of Engineering |
Department |
Electrical & Computer Engineering |
Creator |
Mastrangelo, Carlos H. |
Other Author |
Williams, L. D.; Ghosh, T. |
Title |
Probing protein binding spectra with fourier microfluidics |
Date |
2010 |
Description |
New developments in microfluidic chip technology enable the construction of chemical spectrum analyzers that can probe the binding interactions between chemical entities. In this paper we report the implementation of a microfluidic chip suitable for Fourier transform measurements of biochemical interactions. The chip consists of a chemical signal generator, a flow cell and a binding sensor surface. The microfluidic signal generator produces a periodic stream of protein plugs in solution flowing at constant velocity through the cell. This flow produces periodic association and dissociation cycles of the protein to a functionalized gold sensing surface placed inside the cell. The sensor activity corresponding to the phasor response of the chemical interaction at the excitation frequency is measured optically using surface plasmon resonance (SPR) imaging. We demonstrated the feasibility of the technique using a model system of carbonic anhydrase-II (CA-II) and immobilized 4-(2- Aminoethyl) benzenesulfonamide (ABS) ligand. The observed transfer function showed a dominant pole at 10.2 mHz corresponding to association and dissociation constants of 4.8 x 103 M-1·s-1, and 3.5 x 10-2 s-1 respectively. |
Type |
Text |
Publisher |
Institute of Electrical and Electronics Engineers (IEEE) |
First Page |
5318 |
Last Page |
5321 |
Language |
eng |
Bibliographic Citation |
Mastrangelo, C. H., Williams, L. D., & Ghosh, T. (2010). Probing protein binding spectra with fourier microfluidics. Conference Proceeding IEEE Engineering in Medicine and Biology Society, 5318-21. |
Rights Management |
(c) 2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE. |
Format Medium |
application/pdf |
Format Extent |
1,021,776 bytes |
Identifier |
ir-main,15609 |
ARK |
ark:/87278/s6rn3sh8 |
Setname |
ir_uspace |
ID |
707396 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6rn3sh8 |