Publication Type |
Journal Article |
School or College |
College of Science; School of Medicine |
Department |
Internal Medicine; Oncological Sciences; Human Genetics; Biology |
Program |
Institute of Human Genetics; Howard Hughes Medical Institute (HHMI) |
Creator |
Capecchi, Mario R.; Bernstein, Kenneth E.; Thomas, Kirk R. |
Other Author |
Bunting, Michaeline; Greer, Joy M. |
Title |
Targeting genes for self-excision in the germ line |
Date |
1999-06-15 |
Description |
A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker Neor, and the two genes flanked with loxP elements. This cassette was targeted to the Hoxa3 gene in mouse ES cells that were in turn used to generate chimeric mice. In these chimeras, somatic cells derived from the ES cells retained the cassette, but self-excision occurred in all ES-cell-derived sperm. |
Type |
Text |
Publisher |
Cold Spring Harbor Lab Press |
Volume |
13 |
Issue |
12 |
First Page |
1524 |
Last Page |
1528 |
Subject |
Cre-recombinase; Hoxa3; Chimeric mice |
Subject MESH |
DNA Damage; Gene Targeting; Integrases |
Language |
eng |
Bibliographic Citation |
Bunting M, Bernstein K E ,Greer J M, Capecchi genes for self-excision in the germ line. Genes Dev. 1999 Jun 15;13(12):1524-8. Retrieved on September 12, 2006 http://www.genesdev.org/cgi/content/full/13/12/1524. |
Identifier |
ir-main,369 |
ARK |
ark:/87278/s6xs6d2z |
Setname |
ir_uspace |
ID |
707116 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6xs6d2z |