Description and validation of a novel real-time RT-PCR enterovirus assay

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Hymas, Weston C.; Hillyard, David R.
Other Author Aldous, Wade K.; Taggart, Edward W.; Stevenson, Jeffery B.
Title Description and validation of a novel real-time RT-PCR enterovirus assay
Date 2008
Description Enteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal control and incorporates modified nucleotide chemistry.
Type Text
Publisher American Association for Clinical Chemistry
Volume 54
Issue 2
First Page 406
Last Page 413
Subject RT-PCR enterovirus assay; Rotbart enterovirus amplicon; Aseptic meningitis
Subject LCSH Enteroviruses; Microbiological assay
Language eng
Bibliographic Citation Hymas, W. C., Aldous, W. K., Taggart, E. W., Stevenson, J. B., & Hillyard, D. R. (2008). Description and validation of a novel real-time RT-PCR enterovirus assay. Clinical Chemistry, 54(2), 406-13.
Rights Management (c)American Association for Clinical Chemistry
Format Medium application/pdf
Format Extent 427,362 bytes
Identifier ir-main,11513
ARK ark:/87278/s6th9586
Setname ir_uspace
ID 706820
Reference URL https://collections.lib.utah.edu/ark:/87278/s6th9586
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