Publication Type |
Journal Article |
School or College |
College of Science |
Department |
Biology |
Creator |
Olivera, Baldomero M. |
Other Author |
Ferro, Ari M.; Higgins, N. Patrick |
Title |
Poly(ADP-ribosylation) of a DNA topoisomerase |
Date |
1983 |
Description |
A DNA topoisomerase activity, copurifying with poly(ADP-ribose) synthetase from calf thymus, >95% inhibited if extensive poly(ADP-ribosylation) is allowed to occur. The inhibited DNA topoisomerase, which has drastically different elution properties on hydroxylapatite, can be reactivated by mild alkaline treatment. These results are consistent with a poly(ADP-ribosylation) of the DNA topoisomerase and covalent attachment of the poly(ADP-ribose) moieties to the topoisomerase by alkali-labile bonds. |
Type |
Text |
Publisher |
American Society for Biochemistry and Molecular Biology (ASBMB) |
Volume |
258 |
Issue |
10 |
First Page |
6000 |
Last Page |
6003 |
Subject |
Nicotinic acid; ADP-ribosylation; Turnover cycle; Synthetases |
Subject LCSH |
DNA topoisomerases; Pyridine nucleotides; NAD (Coenzyme); Ligases |
Language |
eng |
Bibliographic Citation |
Ferro, A. M., Higgins, N. P., & Olivera, B. M. (1983). Poly(ADP-ribosylation) of a DNA topoisomerase. Journal of Biological Chemistry, 258(10), 6000-3. |
Rights Management |
(c)American Society for Biochemistry and Molecular Biology (ASBMB) http://www.asbmb.org/ |
Format Medium |
application/pdf |
Format Extent |
1,775,393 bytes |
Identifier |
ir-main,8186 |
ARK |
ark:/87278/s6bp0m6n |
Setname |
ir_uspace |
ID |
705429 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6bp0m6n |