Publication Type |
Journal Article |
School or College |
School of Medicine |
Department |
Ophthalmology |
Creator |
Baehr, Wolfgang |
Other Author |
Maeda, Akiko; Maeda, Tadao; Imanishi, Yoshikazu; Kudsa, Vladimir; Alekseev, Andrei; Bronson, Darin J.; Zhang, Houbin; Zhui, Li; Sun, Wenyu; Saperstein, David A.; Rieke, Fred; Palczewski, Krzysztof |
Title |
Role of photoreceptor-specific retinol dehyrogenase (prRDH) in the retinoid cycle in vivo |
Date |
2005 |
Description |
The retinoid cycle is a recycling system that replenishes the 11-cis-retinal chromophore of rhodopsin and cone pigments. Photoreceptor-specific retinol dehydrogenase (prRDH) catalyzes reduction of all-trans-retinal to all-trans-retinol and is thought to be a key enzyme in the retinoid cycle. We disrupted mouse prRDH (human gene symbol RDH8) gene expression by targeted recombination and generated a homozygous prRDH knockout (prRDH-/-) mouse. Histological analysis and electron microscopy of retinas from 6-8 week-old prRDH-/- mice revealed no structural differences of the photoreceptors or inner retina. For brief light exposure, absence of prRDH did not affect the rate of 11-cis-retinal regeneration or the decay of Meta II, the activated form of rhodopsin. Absence of prRDH did, however, cause significant accumulation of all-trans-retinal following exposure to bright lights, and delayed recovery of rod function as measured by electroretinograms and single cell recordings. Retention of all-trans-retinal resulted in slight overproduction of A2E, a condensation product of all-trans-retinal and phosphatidylethanolamine. We conclude that prRDH is an enzyme that catalyzes reduction of all-trans-retinal in the rod outer segment, most noticeably at higher light intensities and prolonged illumination, but is not an essential enzyme of the retinoid cycle. |
Type |
Text |
Publisher |
American Society for Biochemistry and Molecular Biology (ASBMB) |
Subject |
Rhodopsin, Photoreceptors; prRDH (RDH8); All-trans-RDH; A2E; Retinoid cycle; Dark adaptation |
Subject MESH |
Rhodopsin; Eye; Photoreceptors, Vertebrate |
Language |
eng |
Bibliographic Citation |
Maeda A, Maeda T, Imanishi Y, Kuksa V, Alekseev A, Bronson DJ, Zhang H, Zhu L, Sun W, Saperstein DA, Rieke F, Baehr W, Palczewski K. (2005). Role of photoreceptor-specific retinol dehyrogenase (prRDH) in the retinoid cycle in vivo. J Biol Chem |
Rights Management |
(c)American Society for Biochemistry and Molecular Biology (ASBMB) |
Format Medium |
application/pdf |
Identifier |
ir-main,256 |
ARK |
ark:/87278/s6sj242g |
Setname |
ir_uspace |
ID |
705471 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6sj242g |