| Publication Type |
Journal Article |
| School or College |
College of Science; School of Medicine |
| Department |
Oncological Sciences; Biology; Human Genetics |
| Program |
Institute of Human Genetics; Howard Hughes Medical Institute (HHMI) |
| Creator |
Capecchi, Mario R. |
| Other Author |
Sharp, J. D.; Capecchi, Nancy E |
| Title |
Altered enzymes in drug-resistant variants of mammalian tissue culture cells. |
| Date |
1973-11 |
| Description |
Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in theenzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8.) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variantscan arise from structural gene mutations. |
| Type |
Text |
| Publisher |
National Academy of Sciences of the United States of America. |
| Volume |
70 |
| Issue |
11 |
| First Page |
3145 |
| Last Page |
3149 |
| Subject |
Drug Resistance; Azaguanine; Clone Cells; Hypoxanthines |
| Subject MESH |
Genes; Mutation; Pentosyltransferases; Nitrosoguanidines |
| Language |
eng |
| Bibliographic Citation |
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9: Sharp JD, Capecchi NE, Capecchi MR, Altered enzymes in drug-resistant variants of mammalian tissue culture cells. Retrieved on September 15.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4522295. |
| Rights Management |
Copyright 1973 National Academy of Sciences of the United States of America. All right Reserved |
| Format Medium |
application/pdf |
| Identifier |
ir-main,423 |
| ARK |
ark:/87278/s6p27gd7 |
| Setname |
ir_uspace |
| ID |
703888 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6p27gd7 |
