| Publication Type |
Journal Article |
| School or College |
School of Medicine |
| Department |
Ophthalmology |
| Creator |
Baehr, Wolfgang |
| Other Author |
Applebury, M. L.; Morita, E. A.; Swanson, R. J. |
| Title |
Characterization of bovine rod outer segment G-protein |
| Date |
1982 |
| Description |
A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks. The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments. The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W., Devlin, M. J. & Applebury, M. L. (1979) J. Biol. Chem 254, 11669-11677). Here we report the characterization and properties of the rod outer segment G-protein, a complex of three subunits a, B, and |
| Type |
Text |
| Publisher |
American Society for Biochemistry and Molecular Biology (ASBMB) |
| Volume |
257 |
| Issue |
11 |
| First Page |
6452 |
| Last Page |
6460 |
| Subject |
Heterotrimeric GTP-Binding Proteins; Macromolecular Substances; Rhodopsin |
| Subject MESH |
Eye Proteins; Photoreceptors; Rod Outer Segments |
| Language |
eng |
| Bibliographic Citation |
Baehr W, Morita EA, Swanson RJ, Applebury ML. (1982). Characterization of bovine rod outer segment G-protein. J Biol Chem, 257(11), 6452-60 |
| Rights Management |
(c)American Society for Biochemistry and Molecular Biology (ASBMB) |
| Format Medium |
application/pdf |
| Identifier |
ir-main,1710 |
| ARK |
ark:/87278/s6zp4qqq |
| Setname |
ir_uspace |
| ID |
706549 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6zp4qqq |
