Publication Type |
Journal Article |
School or College |
School of Medicine |
Department |
Ophthalmology |
Creator |
Baehr, Wolfgang |
Other Author |
Imanishi, Yoshikazu; Batten, Matthew L.; Piston, David W.; Palczewski, Krzysztof |
Title |
Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye |
Date |
2004 |
Description |
Visual sensation in vertebrates is triggered when light strikes retinal photoreceptor cells causing photoisomerization of the rhodopsin chromophore 11-cis-retinal to all-trans-retinal. The regeneration of preillumination conditions of the photoreceptor cells requires formation of 11-cis-retinal in the adjacent retinal pigment epithelium (RPE). Using the intrinsic fluorescence of all-trans-retinyl esters, noninvasive two-photon microscopy revealed previously uncharacterized structures (6.9 +/- 1.1 microm in length and 0.8 +/- 0.2 microm in diameter) distinct from other cellular organelles, termed the retinyl ester storage particles (RESTs), or retinosomes. These structures form autonomous all-trans-retinyl ester-rich intracellular compartments distinct from other organelles and colocalize with adipose differentiation-related protein. As demonstrated by in vivo experiments using wild-type mice, the RESTs participate in 11-cis-retinal formation. RESTs accumulate in Rpe65-/- mice incapable of carrying out the enzymatic isomerization, and correspondingly, are absent in the eyes of Lrat-/- mice deficient in retinyl ester synthesis. These results indicate that RESTs located close to the RPE plasma membrane are essential components in 11-cis-retinal production. |
Type |
Text |
Publisher |
Rockefeller University Press |
Volume |
164 |
Issue |
3 |
First Page |
373 |
Last Page |
383 |
Subject |
Microscopy, Fluorescence; Visual Perception; Membrane Proteins |
Subject MESH |
Cytoplasmic Vesicles; Pigment Epithelium of Eye; Retinaldehyde |
Language |
eng |
Bibliographic Citation |
Imanishi Y, Batten ML, Piston DW, Baehr W, Palczewski K. (2004). Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye. J Cell Biol, 164(3), 373-83 |
Rights Management |
(c) Rockefeller University Press |
Format Medium |
application/pdf |
Identifier |
ir-main,1735 |
ARK |
ark:/87278/s64b3k0c |
Setname |
ir_uspace |
ID |
707130 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s64b3k0c |