| Publication Type |
Journal Article |
| School or College |
College of Science; School of Medicine |
| Department |
Internal Medicine; Neurobiology & Anatomy; Oncological Sciences; Human Genetics; Biology |
| Program |
Institute of Human Genetics; Howard Hughes Medical Institute (HHMI) |
| Creator |
Capecchi, Mario R.; Mansour, Suzanne L.; Thomas, Kirk R. |
| Title |
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. |
| Date |
1988-11-24 |
| Description |
Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene. |
| Type |
Text |
| Publisher |
Nature Publishing Group |
| Volume |
336 |
| Issue |
6197 |
| First Page |
348 |
| Last Page |
352 |
| Subject |
Animals; Clone Cells; Restriction Mapping; Stem Cells |
| Subject MESH |
Mice, Transgenic; Mutation; Proto-Oncogenes; Transfection |
| Language |
eng |
| Bibliographic Citation |
Nature. 1988 Nov 24;336(6197):348-52. Mansour SL, Thomas KR, Capecchi MR. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Retrieved on November 1 2006 from http://www.nature.com/nature/journal/v336/n6197/abs/336348a0.html. |
| Rights Management |
copyright © 1988 Nature Publishing Group. All Rights Reserved. |
| Format Medium |
application/pdf |
| Identifier |
ir-main,519 |
| ARK |
ark:/87278/s60p1hnh |
| Setname |
ir_uspace |
| ID |
706924 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s60p1hnh |
