| Publication Type |
Journal Article |
| School or College |
College of Science; School of Medicine |
| Department |
Oncological Sciences; Biology; Human Genetics |
| Program |
Institute of Human Genetics; Howard Hughes Medical Institute (HHMI) |
| Creator |
Thomas, Kirk R.; Capecchi, Mario R. |
| Other Author |
Deng, Chuxia |
| Title |
High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. |
| Date |
1992-07 |
| Description |
Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished. |
| Type |
Text |
| Publisher |
American Society for Microbiology |
| Volume |
12 |
| Issue |
7 |
| First Page |
2919 |
| Last Page |
2923 |
| Subject |
Drug Resistance; Gene Rearrangement; Gentamicins |
| Subject MESH |
Genetic Vectors; Mutagenesis, Site-Directed; Stem Cells |
| Language |
eng |
| Bibliographic Citation |
Mol Cell Biol. 1992 Jul;12(7):2919-23: Thomas KR, Deng C, Capecchi MR. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. Retrieved on September 15.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1620105. |
| Rights Management |
Copyright © 1992, American Society for Microbiology. All Rights Reserved. |
| Format Medium |
application/pdf |
| Identifier |
ir-main,425 |
| ARK |
ark:/87278/s6vh66dk |
| Setname |
ir_uspace |
| ID |
706429 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6vh66dk |
