Hormone sensitivity of particulate pineal guanylate cyclase

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Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Carmack, Curtis F.
Title Hormone sensitivity of particulate pineal guanylate cyclase
Date 1986-12
Description Guanylate cyclase was studied in vitro using crude plasma membrane fractions of bovine and rabbit pineals. The particulate quanylate cyclase was stimulated 1.5-to 6-fold by the non-ionic detergent triton X-100. The effects of various adrenergic agents and pineal indoles were also tested. Serotonin, the endogenous precursor to the pineal hormone melatonin, stimulated particulate guanylate cyclase with optimal stimulation at 10-5 M. Melatonin inhibited particulate guanylate cyclase, with a maximal inhibition of 85% at 10-3 M. The adrenergic agents isoproterenol ( a ?-agonist) and phenylephrine (an ?-agonist) inhibited the particulate guanylate cyclase 95% and 75%, respectively. The endogenous adrenergic neurotransmitter, norepinephrine, had no effect on pineal particulate guanylate cyclase. Combinations of ?- and ?-agonists were effective inhibitors at concentrations that had no effect if either agent was added alone. Stimulation of guanylate cyclase by serotonin and inhibition by melatonin imply the possibility that these two endogenous pineal indoles may play a role in feedback control of pineal cGMP metabolism, and possible other pineal functions.
Type Text
Publisher University of Utah
Subject Cyclic guanylic acid; Melatonin; Serotonin; Pineal gland
Subject MESH Cyclic GMP; Melatonin; Serotonin; Pineal Gland
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Hormone sensitivity of particulate pineal guanylate cyclase Spencer S. Eccles Health Sciences Library.
Rights Management © Curtis F. Carmack.
Format Medium application/pdf
Format Extent 601,024 bytes
Identifier undthes,3982
Source Original University of Utah Spencer S. Eccles Health Sciences Library (no longer available)
Master File Extent 601,071 bytes
ARK ark:/87278/s6c82c6p
Setname ir_etd
Date Created 2012-04-24
Date Modified 2018-01-09
ID 191827
Reference URL https://collections.lib.utah.edu/ark:/87278/s6c82c6p