Peroxidase activity in neutrophilic myelocyte (postnatal liver, rat)

Electron microscopy (peroxidase reaction with diaminobenzidin staining). An early neutrophilic myelocyte with a large nucleus and well developed organelles, distinct Golgi areas (3) and granules. The Golgi areas, rough endoplasmic reticulum and nuclear membrane stain positively (species-dependent), as for most of the granules. The primary (1, 2) ('non-specific' or light microscopic azurophilic) granules are densely stained and contain among others acid phosphatase and arylsulfatase. Secondary (specific) granules containing lysozyme and phagocytin are hardly present in this myelocyte.