Melanopsin: Targeted Ectopic Expression for Optogenetic Visual Restoration
Creator
Michael Gilhooley; Moritz Lindner; Steven Hughes; Mark Hankins
Affiliation
(MG) University of Oxford & Moorfields Eye Hospital, Oxford, United Kingdom of Great Britain and Northern Ireland; (ML) Institute of Physiology and Pathophysiology, Philipps University, Marburg, Germany; (SH) (MH) University of Oxford, Oxford, United Kingdom of Great Britain and Northern Ireland
Subject
Genetic Disease; Pupils Retina; Optic Nerve Trauma and Treatment
Description
Expression of melanopsin renders neural cells sensitive to light, and is emerging as a prime candidate for clinical optogenetic approaches to visual restoration. The retinal neural tissue surviving in the inherited retinal degenerations (IRDs) after visual loss presents a model target for such approaches, however it is unclear if targeting specific neuronal populations within this tissue is advantageous over non-specific delivery. A fair comparison between these two methods is made difficult by the persistence of native photosensitive retinal ganglion cell (pRGC) responses in IRD models. Here we describe a novel model lacking all native retinal light responses to allow a more robust comparison of optogenetic targets. The purpose of our study is to test the hypothesis that retinae devoid of both canonical and pRGC photoreception will remove the 'noise' of intrinsic responses and allow comparison of targeted expression of melanopsin in ON-bipolar cells with non-specific delivery.
Date
2019-03
Language
eng
Format
video/mp4
Type
Image/MovingImage
Source
2019 North American Neuro-Ophthalmology Society Annual Meeting
Relation is Part of
NANOS 2019: Scientific Platform III
Collection
North American Neuro-Ophthalmology Association. NANOS Executive Office 5841 Cedar Lake Road, Suite 204, Minneapolis, MN 55416
Publisher
Spencer S. Eccles Health Sciences Library, University of Utah
Holding Institution
North American Neuro-Ophthalmology Association. NANOS Executive Office 5841 Cedar Lake Road, Suite 204, Minneapolis, MN 55416