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TitleDescriptionSubjectCollection
526 Cortex of lymph node (human)Stain: Azan. The cortex of the lymph node comprises the (secondary) follicles with germinal centres (1) and corona or mantle zone (2), all filled with B lymphocytes. The paracortical area (3) below the follicles largely comprises T cells. Afferent lymph vessels enter via the capsule (6) into the m...cortex; paracortexPoja Histology Collection - Lymphatic Tissues and Organs Subset
527 Cystic corpuscle in thymus (human, adult)Stain: Azan. With age the remaining Hassall's corpuscles become more prominent and might represent large multiform structures of over hundreds of microns. The central cells (*) are keratinized. They might be swollen, calcify and become necrotic eventually, or undergo lysis, leaving a large cystic st...thymic corpuscle (Hassalls); epithelioreticular cell (ERC); lymphoid tissuePoja Histology Collection - Lymphatic Tissues and Organs Subset
528 Cystic corpuscle in thymus (mouse, young adult)Electron microscopy. This cystic corpuscle is lined by specialized epithelioreticular cells and exhibits a lumen (1) which is filled with long microvilli however, curiously derived from a flattened epithelial cell (2, nucleus). The cell contains few electron-dense keratohyalin granules (3). (-->) po...epithelioreticular cells; thymic corpuscle; thymic cyst; lymphoid tissuePoja Histology Collection - Lymphatic Tissues and Organs Subset
529 Dendritic cells in spleen (mouse)Electron microscopy. The interdigitations (*) of the left dendritic cells (antigen-presenting cell or APC) (1 and 2a) are clearly shown. The right (2b) is a neighbour dendritic cell sectioned at the level of the Golgi area. (3) lymphocyte and (4) reticular cell.electron microscopy; dendritic cellPoja Histology Collection - Lymphatic Tissues and Organs Subset
530 Dendritic cells in spleen (rat)Electron microscopy. The interdigitating dendritic cells (1) (so-called antigen-presenting cell, APC) exhibit numerous slender cell projections (1). (2) shows a macrophage with large lysosomes with heterogeneous contents. Small elongated fibroblastic reticular cells (3) form a structural framework ...electron microscopy; dendritic cellPoja Histology Collection - Lymphatic Tissues and Organs Subset
531 Detail of pharyngeal tonsils (human)Stain: Azan. In contrast to the palatine and lingual tonsils, the epipharyngeal tonsil has a ciliated epithelium (1). Islands of multilayered squamous epithelium may interrupt it (transition zone, 2). The epithelium may be infiltrated with lymphocytes. The surface of this tonsil can be enlarged by m...pharyngeal tonsil; GALT; squamous epithelium; ciliated epitheliumPoja Histology Collection - Lymphatic Tissues and Organs Subset
532 Detailed localization of heparan sulfate (HS) in spleen (rat)Stain: Immunofluorescence of Alexa 594 red labeled single chain antibody 3C3 for heparan sulfate (HS). The antibody stains HS epitopes of the meshwork of reticulum cells and the basal membrane of blood vessels. A: survey white pulp spleen. B: marginal sinuses between PALS area and red pulp. C: red...white pulp; PALS; heparan sulfatePoja Histology Collection - Lymphatic Tissues and Organs Subset
533 The effect of cyclophosphamide on B cells in spleen (rat)Stain: Immunofluorescence of Vector red using Mark-1 antibody against B cells. A: Normal rat spleen. (1) the dark, unstained area represents the PALS (periarteriolar lymphatic sheath) filled with T cells. (2) red-stained germinal centre of a B cell follicle that is surrounded by the marginal zone (3...cyclophosphamide; immunosuppression; immunofluorescence; B lymphocytesPoja Histology Collection - Lymphatic Tissues and Organs Subset
534 The effect of cyclophosphamide on CD3-thymocytes in thymus (rat)Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section.. A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the dark-blue stained cortex and the lightly stained medulla in normal thy...cyclophosphamide; CD3 monoclonal antibody; lymphoid tissue ; immunosuppressionPoja Histology Collection - Lymphatic Tissues and Organs Subset
535 The effect of cyclophosphamide on splenic B cells (rat)Stain: Immunoperoxidase staining using diaminobenzidin (DAB)/ hematoxylin counterstained on frozen section of B cells with the antibody Mark-1. The PALS area (1) contains T cells and remains unstained blue. The positively stained B cells (brown) are found in the germinal centres (2) and in the coron...cyclophosphamide; immunosupression; B lymphocytes; Mark 1 antibodyPoja Histology Collection - Lymphatic Tissues and Organs Subset
536 The effect of cyclophosphamide on the CD8-thymocytes in thymus (rat)Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section. A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the darkly stained cortex and the lightly stained medulla in normal thymus ...cyclophosphamide; CD8 monoclonal antibody; immunosuppression; lymphoid tissuePoja Histology Collection - Lymphatic Tissues and Organs Subset
537 The effect of cyclophosphamide on the resident macrophages in thymus (rat)Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section. A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days. The darkly stained cortex of th normal thymus (A1, A2) decreases its dark stain...cyclophosphamide; ED1 macrophages ; immunosuppression; lymphoid tissuePoja Histology Collection - Lymphatic Tissues and Organs Subset
538 The effect of cyclophosphamide treatment on the B and T cells in spleen (rat)Stain: Immunoperoxidase staining using diaminobenzidin (DAB)/ hematoxylin counterstained on frozen section with antibodies to B cells (Mark 1), CD3 and CD8 T cells. (A): B cells in the follicles, germinal centres (2) and corona are stained positive brown, while the PALS area (1) is negative (blue)...CD3 lymphocytes; CD8 lymphocytes; B lymphocytes; cyclophosphamidePoja Histology Collection - Lymphatic Tissues and Organs Subset
539 Follow-up of the process of diapedesis through stratified epithelium of the palatine tonsil (gut-associated lymphatic tissue or GALT) (human)Stain: (A, C) Azan and (B, D) anti-keratin-antibody and immunoperoxidase staining using diaminobenzidin (DAB) on frozen section, counterstained with hematoxylin. (1) (keratinized) stratified squamous epithelial cells. (2) between desquamated epithelial cells the spaces are filled with infiltrated l...stratified squamous epithelial cells; GALTPoja Histology Collection - Lymphatic Tissues and Organs Subset
540 Hassall's corpuscle in thymus (human, puberty)Stain: Hematoxylin. A larger magnification of the thymic medulla reveals the purple-stained structures in the centre of a so-called Hassall's (or thymic) corpuscle consisting of keratinized (*) epithelial cells. It is surrounded by recognizable flattened cells showing keratohyalin granules (-->). T...thymic corpuscle (Hassalls); epithelioreticular cell (ERC); lymphoid tissuePoja Histology Collection - Lymphatic Tissues and Organs Subset
541 Hassall's corpuscle in thymus (mouse)Electron microscopy. The centre of a small Hassall's corpuscle consists of darker-stained cells (1) which are keratinizing and represent degenerating cytoplasmic remnants (2). (3) is an infiltrating monocyte and (*) indicate the presence of free keratohyalin granules from disintegrated epithelial ce...thymic corpuscle (Hassalls); lymphoid tissue ; epithelioreticular cell (ERC)Poja Histology Collection - Lymphatic Tissues and Organs Subset
542 High endothelial venules (HEV) (rat lymph node, human palatine tonsil)Stain: (A) Anti-laminin-antibody and immunoperoxidase staining using diaminobenzidin (DAB)/ hematoxylin counterstained on frozen section of lymph node; (B) electron microscopy (palatine tonsil). (A): Using an antibody against laminin brown-stained basement membranes are outlined demonstrating postc...palatine tonsil; high endothelial venule (HEV); laminin; immunohistochemistryPoja Histology Collection - Lymphatic Tissues and Organs Subset
543 Hilum side of lymph node (human)Stain: Azan. Site of the hilum of a lymph node includes part of the medulla and medullary cords (1) and medullar sinuses (2). In the hilum (H) dilated efferent lymphatic vessels (3) and draining veins (4) and entering arteries (5) are present. (6) represents adipose tissue embedding the efferent ves...hilum; lymphatic vessels; medullary cords; paracortexPoja Histology Collection - Lymphatic Tissues and Organs Subset
544 Ileum with Peyer's patches ('gut-associated lymphatic tissue' or GALT) (human)Stain: Azan. Survey ileum (see also Digestive System: Ileum) A large amount of non-encapsulated diffuse lymphatic tissue or mucosa-associated lymphatic tissue (MALT) is located in the subepithelial lamina propria/submucosa of the ileum and called 'gut-associated lymphatic tissue' (GALT). These so-c...follicle; lymph node; germinal centerPoja Histology Collection - Lymphatic Tissues and Organs Subset
545 Immunohistochemical identification of splenic B cells (rat)Stain: Immunofluorescence of FITC-labeled anti-Mark-1 antibody against rat B cells, carried out on spleen cells after fixation (A, B) or non-fixated spleen cells (C). (A) shows (young) plasma cells producing antibodies. (B) a green-stained B cell. The surrounding negative cells are likely T cells....immunofluorescence; B lymphocyte; cappingPoja Histology Collection - Lymphatic Tissues and Organs Subset
546 Immunohistochemistry of B cells in splenic white pulp (rat)Stain: Immunohistochemistry of Vector red for Mark-1-antibody-stained B cells. (1) indicates the PALS area populated with unstained T cells located around the central artery of the white pulp. (2) indicates the positively red stained B cells in the germinal centre of the follicle. (3) the B-cells i...marginal zone; immunohistochemistry; folliclePoja Histology Collection - Lymphatic Tissues and Organs Subset
547 Immunohistochemistry of ED1-positive subset of macrophages in spleen (rat)Stain: Immunoperoxidase staining using diaminobenzidin (DAB)/ hematoxylin counterstained on frozen section with the anti-macrophage antibody ED1. Most of the labeled (brown) macrophages are found in the red pulp (2) up to the marginal zone border (B). The PALS area (1) contains sparsely spread ED1 ...ED1 ; macrophages; immunohistochemistry; marginal zonePoja Histology Collection - Lymphatic Tissues and Organs Subset
548 Immunohistochemistry of laminin in cortex lymph node (rat)Anti-laminin-antibody and immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section. Using an antibody against laminin brown-stained basement membranes (BM) are outlined demonstrating postcapillary venules (1) in the paracortical areas, as well as larger b...paracortex; high endothelial venule (HEV); laminin; immunohistochemistryPoja Histology Collection - Lymphatic Tissues and Organs Subset
549 Immunohistochemistry of thymus (rat)Stain: Alexa-594 red immunofluorescence. (1) Medulla. (2) Cortex. (A): Staining with a monoclonal antibody against vimentin illustrates that vimentin is localised in the stromal cells of the connective tissue of the capsule, of the septa or trabeculae that invade the cortex (2) and overwhelmingly ...vimentin; laminin; fibronectin; ED2 monoclonal antibodyPoja Histology Collection - Lymphatic Tissues and Organs Subset
550 Immunohistochemistry with cellular markers in thymus (rat)Stain: Alexa-594 red immunofluorescence. (1) medulla. (2) cortex. (A-survey, B-cortex): ER13 antibody stains for MHC-class II antigens on reticular cell types in medulla and cortex. (C): ED1 monoclonal antibody stains a single chain glycoprotein of 110 kDa on the lysosomal membrane of myeloid cel...ER13 antibody; ED1 monoclonal antibody; ED2 monoclonal antibody; ER2 monoclonal antibodyPoja Histology Collection - Lymphatic Tissues and Organs Subset
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