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- Chapter 1 focuses on the inhibition of the β-catenin/B-Cell lymphoma 9 proteinprotein interaction. This protein-protein interaction is found in Wnt signaling and has been strongly linked to cancer. To inhibit the interaction, molecules were computationally designed, synthesized and tested. A series of 33 final compounds was synthesized, resulting in a lead molecule with an inhibition constant of 5.2 μM. The binding mode of this molecule was validated through the structure activity relationship of the series as well as mutational studies. The lead molecule was shown to exhibit 98-fold selectivity for the β-catenin/B-Cell lymphoma 9 interaction over the β-catenin/E-cadherin interaction, inhibit the Wnt pathway in a cell based assay and inhibit the growth of Wnt/β-catenin-dependent cancer cells. Chapter 2 describes an attempt to overexpress and purify the ethylene receptor, a membrane protein found in plants critical to all stages of plant life. There are major unanswered questions involving the ethylene receptor, but most important is the nature of the binding interaction between ethylene and the receptor. We intended to characterize the interaction using a variety of spectral techniques, which would require large amounts of protein. Several strategies to express and purify the receptor were pursued. Chapter 3 lays the foundation for using Stark vibrational spectroscopy to more thoroughly understand the mechanism of proton pumping. Stark vibrational probes were installed in the proton pump ubiquinol oxidase. Functional protein containing the probes iv was successfully expressed and purified. The probes were then utilized to survey the electric field within the proton pump. Changes in electric field were detected in multiple locations and synchronistic results were observed for probes positioned in the same region of the protein. The presented work was highly decoupled and covered a broad area. Completion required efforts in organic synthesis, molecular biology, protein chemistry and spectroscopy. Overall, important foundational achievements were made on several challenging and significant systems.1
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Filters: Collection: "ir_etd"
| Author | Title | Subject | Date | Publication Type | ||
|---|---|---|---|---|---|---|
| 276 |
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Page 171 | ||||
| 277 |
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| 278 |
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| 279 |
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Page 48 | ||||
| 280 |
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Page 65 | ||||
| 281 |
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Page 206 | ||||
| 282 |
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Page 41 | ||||
| 283 |
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Page 93 | ||||
| 284 |
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Page 179 | ||||
| 285 |
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| 286 |
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Page 194 | ||||
| 287 |
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Chalishazar, Milind D. | Metabolic vulnerabilities in MYC-Driven small cell lung cancer | 2019 | dissertation | |
| 288 |
 |
Chen, Ying-Sheng | Effects of bison meat consumption on blood lipids and selective biomarkers related to cancer risk | Buffalo meat; Beef; Cancer | 2009-05 | thesis |
| 289 |
 |
Olson, Kristofor Aksamit | Mitochondrial pyruvate metabolism in cancer and stem cells of the colon epithelium | 2016-05 | dissertation | |
| 290 |
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Page 174 | ||||
| 291 |
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Page 42 | ||||
| 292 |
 |
Beamer, Laura Curr | Quality of Life Among Women With Radiation-Induced Dermatitis of the Breast | 2016-05 | dissertation | |
| 293 |
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Page 134 | ||||
| 294 |
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Page 157 | ||||
| 295 |
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Page 187 | ||||
| 296 |
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Page 194 | ||||
| 297 |
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Page 26 | ||||
| 298 |
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Page 37 | ||||
| 299 |
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Page 38 | ||||
| 300 |
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Page 38 |