Immunohistochemistry with cellular markers in thymus (rat)

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Title Immunohistochemistry with cellular markers in thymus (rat)
Creator Poels, Lambert G.
Contributor Lambert G. Poels, PhD, UMC St Radboud Nijmegen; Paul H. K. Jap, PhD, UMC St Radboud Nijmegen
Date 2010-06-11
Subject cyclophosphamide; CD8 monoclonal antibody; OX19 monoclonal antibody; ER1 monoclonal antibody
Description Stain: Alexa 594 red immunofluorescence. (1) medulla; (2) cortex; (3) septa. (A): Strong CD8 staining of the thymic cortical lymphocytes (single CD8+ and double positive CD4+CD8+ thymocytes). (B): OX19 staining for almost all T cells, equivalent to T1 human marker and Ly-1 mouse marker. Note that the (mature) medullary T cell show stronger OX-19 expression than the (immature) cortical thymocytes. (C): ER1 antibody stains all T cells being equivalent to CD43. Both cortex and medulla are well stained. (D) ED1 antibody stains resident tissue macrophages which are present more in the well capillarised medulla than in the cortical area.
Subtype Image
Format image/jpeg
Collection Poja Histology Collection - Lymphatic Tissues and Organs Subset
ARK ark:/87278/s6hh9n9n
Setname ehsl_heal
ID 890956
Reference URL https://collections.lib.utah.edu/ark:/87278/s6hh9n9n
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