The effect of cyclophosphamide on the CD8-thymocytes in thymus (rat)

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Title The effect of cyclophosphamide on the CD8-thymocytes in thymus (rat)
Creator Poels, Lambert G.
Contributor Lambert G. Poels, PhD, UMC St Radboud Nijmegen; Paul H. K. Jap, PhD, UMC St Radboud Nijmegen
Date 2010-06-11
Subject cyclophosphamide; CD8 monoclonal antibody; immunosuppression; lymphoid tissue
Description Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section. A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the darkly stained cortex and the lightly stained medulla in normal thymus (A1, A2) turn to darkly stained medulla and a lightly stained cortex (B1, B2) four days after a single injection with CP. The thymus recovers to normal about 10 days later. (C) shows a negative immunoperoxidase control of a normal thymus. The anti-CD8 monoclonal antibody in normal thymus tissue (A1, A2) shows an intense cortical staining, while in the medulla (A2-1) the positively stained cells are much less, since the medulla contains single-positive CD8 cells, while the cortex contains both double-positive (CD4+CD8+) and single positive lymphocytes (CDE8+). The large CD4+ CD8+ population harbours the majority of dividing thymocytes. Due to the CP-induced inhibition of cell proliferation in the cortex (B1, 2) the remaining cortical thymoblasts hardly express CD8, while the medullar thymocytes are strongly positive, confirming the so-called transient thymic inversion. (A1, A2): Normal thymus. (B1, B2): CP-treated thymus. (C): A negative immunoperoxidase control. (1) medulla; (2) cortex; (3) septa or trabeculae.
Subtype Image
Format image/jpeg
Rights https://rightsstatements.org/page/CNE/1.0/
Collection Poja Histology Collection - Lymphatic Tissues and Organs Subset
ARK ark:/87278/s6865jsw
Setname ehsl_heal
ID 890927
Reference URL https://collections.lib.utah.edu/ark:/87278/s6865jsw
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