The effect of cyclophosphamide on CD3-thymocytes in thymus (rat)

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Title The effect of cyclophosphamide on CD3-thymocytes in thymus (rat)
Creator Poels, Lambert G.
Contributor Lambert G. Poels, PhD, UMC St Radboud Nijmegen; Paul H. K. Jap, PhD, UMC St Radboud Nijmegen
Date 2010-06-11
Subject cyclophosphamide; CD3 monoclonal antibody; lymphoid tissue ; immunosuppression
Description Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstained on frozen section.. A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the dark-blue stained cortex and the lightly stained medulla in normal thymus (A1, A2) turn to darkly stained medulla and a lightly stained cortex (B1, B2) four days after a single injection with CP. The thymus recovers to normal about 10 days later. (C) shows a negative immunoperoxidase control of a normal thymus. The anti-CD3 monoclonal antibody shows low staining in the normal cortex (A1-2), but medullar thymocytes are CD3+ (A1-1 and A2-1). CD3 expression increases on all T cells upon maturation of the cells as they migrate towards the medulla. Figure B1 and B2 show that, after CP treatment the strong CD3+ expression in the medulla (B1-1, B2-1) remains unaltered since these non-mitotic cells are not affected by CP. CD3 remains low in the cortex (B2-2). After ten days (C) the cortex and medulla pattern are repopulated to normal levels as shown in A.
Subtype Image
Format image/jpeg
Rights https://rightsstatements.org/page/CNE/1.0/
Collection Poja Histology Collection - Lymphatic Tissues and Organs Subset
ARK ark:/87278/s6j135g9
Setname ehsl_heal
ID 890863
Reference URL https://collections.lib.utah.edu/ark:/87278/s6j135g9
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