Laminin in the canalicular period of the lung (mouse, embryo)

Stain: Fluorescence microscopy with anti-laminin antibody. Laminin is a component of the basement membrane. Strong fluorescence visualizes the course of all basement membranes, especially around the bronchioles (B) and their branches in the developing lung tissue. In between the distal air spaces (tubes, T) lined by cuboidal epithelium and capillary basement membranes are also stained. At (↓) positive blood vessel within the pleura (PL↔). The anti-laminin antibody is used as a marker for intact or disrupted basement membranes of epithelial structures and tumor metastasis.