Comparative effects of chrysotile asbestos fibers on lavaged pulmonary macrophages.

Publication Type thesis
School or College School of Medicine
Department Family & Preventive Medicine
Author Gallagher, Jane Elizabeth.
Title Comparative effects of chrysotile asbestos fibers on lavaged pulmonary macrophages.
Date 1983-06
Description Chrysotile asbestos is cytotoxic to alveolar macropages. The mechanisms involved in initiating these cytotoxic events are poorly understood. Some investigators believe that the magnesium component of chrysotile is largely responsible. Others attribute the cytotoxicity to be the results of the chrysotile’s physical characteristics. In this project, lavaged alveolar macrophages were used to provide a bioassay for detecting fiver uptake over time. In several separate experiments, two fiber populations differing significantly in both chemical and physical characteristics were added to cultured macrophages. The results suggest that ingestion of magnesium-leached fibers took place to a greater extent than unleached fibers, as evidenced by decreasing numbers of fibers attached to macrophages over time. The surface morphology of alveolar macrophages was not altered significantly by addition of either leached or unleached fibers. In addition, enzyme analysis of two marker enzymes, beta-glucuronidase and lactate dehydrogenase, were not significantly elevated, indicating lack of cytotoxicity over the three-hour period studied. This study provides information useful of future mechanistic investigations on cytopathologic interactions between macrophages and asbestos fibers.
Type Text
Publisher University of Utah
Subject Pulmonary Lavage; Phagocoytes
Subject MESH Asbestosis; Macrophages; Asbestos
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of “Comparative effects of chrysotile asbestos fibers on lavaged pulmonary macrophages.” Spencer S. Eccles Health Sciences Library. Print version of “Comparative effects of chrysotile asbestos fibers on lavaged pulmonary macrophages.” available at J. Willard Marriott Library Special Collection. RC 39.5 1983 G34.
Rights Management © Jane Elizabeth Gallagher.
Format Medium application/pdf
Identifier us-etd2,28
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
ARK ark:/87278/s6np2k1j
ID 193827
setname ir_etd
Reference URL https://collections.lib.utah.edu/details?id=193827
Back to Search Results