Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells.

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Publication Type thesis
School or College School of Medicine
Department Pathology
Author Slhokoohi, Vida.
Title Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells.
Date 1986-12
Description The characteristic of the organisms from gonococcal type 5 morphology and their interaction with human epithelial cells are least studied among different types of gonococci. Organisms from type 5 morphology which were isolated from the fallopian tube of a woman with gonococcal salpingitis exhibited very intense opacity without piliation. After 48 hours of incubation at 37°C with a 2% CO2 environment, organisms from type 5 morphology transformed to type 4 morphology. SDS-PAGE of whole cell lysates of organisms from colonial type 5 revealed presence of a 27kd protein molecule which was absent in the whole cell lysates of the organisms with type 4 morphology, which were obtained as results of colonial type 5 passage. Also silver-stained SDS-PAGE profiles of pronase-treated whole cell lysates of gonococci showed some or all higher peaks in lanes filled with type 4 gonococci than did lanes with type 5 gonococci. This observation suggests that type 4 gonococci may have more lipooligosaccharide or a different form of these molecules than do type 5 gonococci. Attachment to buccal cells of gonococci organisms of colony type 1,4 and 5 differed markedly with the following order: 1>5>4. Colony type 5 gonococci were not piliated, nor were type 4 colonies, so some factor other than pili in type 5 is responsible for the greater attachment of type 5. Since pili are the only attachment factor identified to date, pursuit of the attachment factor used by type 5 gonococci seems warranted.
Type Text
Publisher University of Utah
Subject Pathology; Pathogenicity
Subject MESH Neisseria gonorrhoeae; Mucous Membrane; Gonorrhea
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells." Spencer S. Eccles Health Sciences Library. Print version of "Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells." available at J. Willard Marriott Library Special Collection. QR 6.5 1986 S54.
Rights Management © Vida Shokoohi.
Format Medium application/pdf
Identifier us-etd2,40
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
ARK ark:/87278/s6pg26b8
Setname ir_etd
Date Created 2012-04-23
Date Modified 2012-04-23
ID 193464
Reference URL https://collections.lib.utah.edu/ark:/87278/s6pg26b8