Genome sequences: experimental determination and evolutionary interpretation

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Publication Type dissertation
School or College School of Medicine
Department Human Genetics
Author Cherry, Joshua L.
Title Genome sequences: experimental determination and evolutionary interpretation
Date 1999-08
Description The genome project is an interdisciplinary effort to determine and analyze the sequences of whole genomes. Laboratory techniques presented here may contribute to this effort. One of these techniques involves the use of enzyme-linked fluorescence for detection of DNA on membranes in hybridization experiments. This technique facilitates the automation of hybridization experiments. Another technique involves the use of modified primers in cycle sequencing reactions to eliminate artifacts and improve sequence determination. Introduction of residues into the primer that can not be replicated by DNA polymerase eliminates the possibility of unwanted exponential amplification of side-products. The sequence of the genome of the hypothermophilic archaeon Pyrococcus furiosus, along with the other microbial gnome sequences becoming ailable, has been the basis of inquiries concerning the evolution of histidine biosynthetic operons and the biochemistry and evolution of inteins. These studies provide evidence of lateral transfer of genetic material and recruitment of genes to new functions. Theoretical work is presented concerning the process by which sequences change over evolutionary time. A model is presented that predicts approximate independence of the rate of change from the effective population size, contrary to the accepted understanding of the nearly neutral theory of molecular evolution.
Type Text
Publisher University of Utah
Subject Hybridization; Experiments
Subject MESH Genome; Laboratory Techniques and Procedures
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Genome sequences: experimental determination and evolutionary interpretation". Spencer S. Eccles Health Sciences Library. Print version of "Genome sequences: experimental determination and evolutionary interpretation". available at J. Willard Marriott Library Special Collection. QH9.7 1999 .C44.
Rights Management © Joshua L. Cherry
Format Medium application/pdf
Format Extent 4,203,749 bytes
Identifier undthes,4470
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 4,203,794 bytes
ARK ark:/87278/s61v5gsf
Setname ir_etd
Date Created 2012-04-24
Date Modified 2012-04-24
ID 191040
Reference URL https://collections.lib.utah.edu/ark:/87278/s61v5gsf