The carbohydrate component of a human gamma globulin

1. The carbohydrate components of human gamma-globulin, fraction II-1,2 were identified and quantitatively estimated. 2. Three glycopeptides were isolated from a papain digest of human gamma-globulin and characterized by chemical and physical methods. 3. The amino acid of the three glycopeptides was determined. 4. The three glycopeptides were found to represent a single carbohydrate group which was attached to the protein by a single aspartic acid residue. The carbohydrate group contained 3 galactose, 5 mannose, 2 fucose, 6 to 8 glucosamine, and 2 sialic acid residue. 5. The significance of these findings was discussed in relation to glycol-proteins and antibodies.