Analysis of the Physiological Roles of the Nonsense Mediated mRNA Decay Pathway in Drosophila Melanogaster

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Publication Type dissertation
School or College School of Medicine
Department Human Genetics
Author Chapin, Alex
Title Analysis of the Physiological Roles of the Nonsense Mediated mRNA Decay Pathway in Drosophila Melanogaster
Date 2014-08
Description The nonsense mediated mRNA decay (NMD) pathway is a conserved posttranscriptional mRNA decay pathway that functions to destabilize a variety of naturally occurring target mRNAs. The NMD pathway functions in all eukaryotes and regulates a significant portion of the transcriptome. It is thought that this regulation is critical as inhibition of NMD leads to physiological and developmental defects in all organisms and in the case of more complex organisms, lethality. It is predicted that overexpression of NMD pathway target genes leads to these defects in NMD mutants. Despite the critical nature of this pathway, little is know about how NMD functions in a developmental and physiological context, including which target genes are most critically regulated by NMD and how the overexpression of these targets may mediate the NMD mutant phenotype. To address this knowledge gap, we first use two genome-wide techniques to identify and characterize the kinds of transcripts targeted by NMD in the context of an intact metazoan, Drosophila melanogaster. We then examine more closely the function of one of these target genes, Gadd45, and find that overexpression of this target in NMD mutants may explain important aspects of the NMD mutant phenotype.
Type Text
Publisher University of Utah
Subject MESH Nonsense Mediated mRNA Decay; Drosophila melanogaster; Gene Expression; Growth and Development; Transcription Factor CHOP; Apoptosis; Gene Expression Regulation; Cell Cycle Checkpoints
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Relation is Version of Digital version of Analysis of the Physiological Roles of the Nonsense Mediated mRNA Decay Pathway in Drosophila Melanogaster
Rights Management Copyright © Alex Chapin 2014
Format Medium application/pdf
Format Extent 5,285,947 bytes
Source Original in Marriott Library Special Collections
ARK ark:/87278/s6wq4hqz
Setname ir_etd
Date Created 2019-03-29
Date Modified 2021-05-06
ID 1409626
Reference URL https://collections.lib.utah.edu/ark:/87278/s6wq4hqz
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