T cell depletion in lymph nodes (dog)

Stain: Trichrome (Goldner). Left and right: survey of lymph nodes. A: lymph node after treatment with anti-thymocyte-antiserum (ATS); B: normal, untreated lymph node ATS treatment results in a considerable depletion of the T cells in the paracortical area (2), while also the germinal centre (1) in (A) appears inactivated compared to the secondary follicle in (B). (3) medullar cords; (4) medullar sinuses; (5) marginal sinus in which an afferent lymph vessel drains (*); (5b) paratrabecular sinus; (6) capsule; (7) arteries.