Keratin 7 staining in dysplastic epithelium of a bronchus (human, adult)

Stain: anti-keratin 7 antibody (Pan-Ck 7) immunoperoxidase staining (with aminoethylcarbazole (AEC) substrate). A red-brown staining with AEC indicates a positive reaction for cytokeratin 7. Disturbances in the growth and maturing might result in dysplasia of the epithelium with a changed reaction pattern for among others cytokeratins. In this mild dysplastic bronchial epithelium the reactivity for keratin 7 appears lost completely. This antibody is considered as useful in the study of premalignant changes in complex epithelia (e.g., pseudostratified, squamous stratified epithelia) within the lungs, cervix, etc.