Predentin formation at the cuspal tip in tooth development - bell stage, human, embryo

Stain: Azan. From top to bottom: Stellate reticulum consisting of a network of ectoderm-derived cells; Cell layers of the stratum intermedium; Columnar (presecretory) ameloblasts with their upper side (nuclear area) in close contact with the stratum intermedium, and at the distal side (secretion area) oriented towards predentin (blue); Odontoblasts in a epithelioid arrangement with their secretion area close to the predentin (blue); In close contact with the odontoblasts a network of fibroblast-like cells (so-called pulpal cells) of the dental papilla (future pulp); blood vessels are also present.