Detection and characterization of antibodies against mouse melanoma MB-16 by immunofluorescence

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Title Detection and characterization of antibodies against mouse melanoma MB-16 by immunofluorescence
Publication Type dissertation
School or College School of Medicine
Department Oncological Sciences
Author Kato, Kenneth Hayami
Date 1972-08
Description Tumor-specific antibodies to mouse melanoma MB-16 were detected by Immunofluorescence in the sera of C57BL/6J mice, the inbred host strain. Fixed tumor cells grown in tissue culture on cover-slips were employed as target cells for immunofluorescent tests. Fluorescence of the cytoplasm of tumor cells was an indication of a positive reaction. Antibody was detectable on the 5th post-challenge day in mice injected (6 x 10[5]) s.c. with MB-16 cells isolated from subcutaneous transplants. The antibody reached a maximum titer of 1:4 on day 10 and this titer persisted until the 21st day following the initial injection. Tumors formed on tails of mice injected s.c. with 6 x 10[5] viable tumor cells were removed by tail amputation 35 days post-challenge. Prior to tumor removal, titers gradually increased from 1:4 to 1:16. The incidence of antibody also increased from 80% after 1 week to 100% in the 5th week after the challenge dose. Following tumor removal, titers fell to 1:4 and then gradually declined. Antibody was still detectable in the serum of mice 19 weeks following removal of the tumor. The antibody persisted in the presence of overt metastatic lesions as well as in apparent absence of tumor. Five of nine (56%) of sera from human patients with malignant melanoma were reactive at low titers (1:2) on MB-16 target cells. Six of nine (78%) melanoma sera were positive on human melanoma cells. The antibody reached titers of 1:8. With on exception, the serum from a patient with choriocarcinoma, 26 sera from patients with disorders unrelated to malignant melanoma, and 20 blood bank sera were either non-reactive or non-specific for human melanoma target cells. The specificity of the fluorescent staining reaction was determined by absorption as sera with heterologous antigens (normal and neoplastic cells), one-step inhibition procedures, and test target cells from different tissue culture cell lines. Absorption of sera with surface membrane preparations from MB-16 cells indicated the absence of antibodies to surface antigens.
Type Text
Publisher University of Utah
Subject Mice; Microbiology
Subject MESH Antibodies; Melanoma
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Detection and characterization of antibodies against mouse melanoma MB-16 by immunofluorescence." Spencer S. Eccles Health Sciences Library. Print version of "Detection and characterization of antibodies against mouse melanoma MB-16 by immunofluorescence." available at J. Willard Marriott Library Special Collection. QR6.5 1972 .K3.
Rights Management © Kenneth H. Kato
Format application/pdf
Format Medium application/pdf
Format Extent 3,997,216 bytes
Identifier undthes,5342
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship Grant from the American Caner Society, T-514.
Master File Extent 3,997,237 bytes
ARK ark:/87278/s6cf9rv4
Setname ir_etd
ID 190603
Reference URL https://collections.lib.utah.edu/ark:/87278/s6cf9rv4
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