Induced asymmetry in lipid bilayers detected by sum frequency vibrational spectroscopy

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Title Induced asymmetry in lipid bilayers detected by sum frequency vibrational spectroscopy
Publication Type thesis
School or College College of Science
Department Chemistry
Author Cooper, Michael P.
Date 2010-08
Description Phospholipids, a major constituent of cell membranes, contain a hydrophobic tail and a hydrophilic head group. Cell membranes are composed of two leaflets forming a bilayer. The hydrophobic tails form the bilayer core while the hydrophilic headgroups form the exterior of the bilayer. While phospholipids have a similar structure in general, there is variety among headgroup moieties as well as tail moieties. In living cells, phospholipids are distributed asymmetrically between the two leaflets of the bilayer according to head group chemistry. However, it has been shown that phospholipids can undergo rapid flip-flop between the two leaflets, which would favor a symmetric distribution due to mixing. Sum frequency vibrational spectroscopy (SFVS), a surface specific technique, can be used to determine the amount of asymmetry in a lipid bilayer on a planar support. As such, SFVS was used to study one possible mechanism of bilayer asymmetry maintenance, namely electrostatic association. Specifically, asymmetry was induced in bilayers consisting of 1,2-distearoyl-sn-glycero-3-phosphatidylcholine and 1,2-distearoyl-sn-glycero-3-phosphatidylserine by electrostatic interaction with the positively charged polypeptide polylysine. Lipid bilayers were created on planar silica surfaces through the Langmuir-Blodgett/Langmuir-Schaefer deposition method. To confirm the validity of using the Langmuir-Blodgett/Langmuir-Schaefer deposition method for the asymmetry study, mass spectrometry was used to verify bilayers of a known composition that were formed on a silica substrate.
Type Text
Publisher University of Utah
Subject Induced asymmetry; Asymmetric synthesis; Bilayer; DSPC; DSPS; Spectroscopy; Sum frequency vibrational spectroscopy; Phospholipids; Bilayer lipid membranes
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Rights Management Copyright © Michael P. Cooper 2010
Format application/pdf
Format Medium application/pdf
Format Extent 3,253,111 bytes
Source Original housed in Marriott Library Special Collections, QD3.5 2011 .C66
ARK ark:/87278/s6571sqs
Setname ir_etd
ID 194324
Reference URL https://collections.lib.utah.edu/ark:/87278/s6571sqs
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