Method used to isolate secretory component from secretory immunoglobulin A

Update Item Information
Title Method used to isolate secretory component from secretory immunoglobulin A
Publication Type thesis
School or College College of Nursing
Department Nursing
Author McCance, Kathryn L.
Date 1974-06
Description An attempt was made to isolate secretory component from the molecule secretory Immunoglobulin A. Two methods were used, each with colostrum as the source of secretory component. In the first method, colostrum was centrifuged, and the supernatant was chromatographed on a Sephadex G-200 column. Half of the eluates were concentrated by negative pressure dialysis, and half of the eluates were chromatographed on a carboxymethyl-50 cation exchange column. All eluates collected were subjected to Immunoelectrophoresis. In the second method the colostrum was clarified, and the proteins were precipitated by the addition of ammonium sulfate. The eluates were chromatographed on a DEAE cellulose column using a stepwise elution of three sodium phosphate buffers. Further chromatography included a GM-50 cation exchange column and identification of the proteins by the method of Ouchterlony using rabbit antiserum against secretory IgA. Free or unattached secretory component was found in the second elution peaks from the Sephadex G-200 gel filtration column and from the first fraction of colostral whey eluted using O.OIM sodium phosphate buffer, at pH 7.6. Secretory component was highly contaminated with lactoferrln and was not obtained In a pure state. Failure to obtain spurring between second peak eluates and antl S-IgA could be the result of differences in quarternary structure between the tetramerlc free form and the IgA bound form.
Type Text
Publisher University of Utah
Subject Colostrum; Immunoelectrophoresis
Subject MESH Immunoglobulin Fragments; Immunoglobulin A
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Method used to isolate secretory component from secretory immunoglobulin A." Spencer S. Eccles Health Sciences Library. Print version of "Method used to isolate secretory component from secretory immunoglobulin A." available at J. Willard Marriott Library Special Collection. QR6.5 1974 .M3.
Rights Management © Kathryn L. McCance.
Format application/pdf
Format Medium application/pdf
Format Extent 627,361 bytes
Identifier undthes,4959
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 627,404 bytes
ARK ark:/87278/s6pr7xr0
Setname ir_etd
ID 191004
Reference URL https://collections.lib.utah.edu/ark:/87278/s6pr7xr0
Back to Search Results