Ganglion Cell Layer Thickness Variance Using SPECTRALIS Optical Coherence Tomography

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Title Ganglion Cell Layer Thickness Variance Using SPECTRALIS Optical Coherence Tomography
Creator Paul Mallory; Sushant Wagley; John Chen; Collin M. McClelland; Peter A. Downie; Bruce Lindgren; Michael S. Lee
Affiliation Department of Ophthalmology and Visual Neuroscience (PM, SW, CMM, PAD, MSL), University of Minnesota, Minneapolis, Minnesota; Departments of Ophthalmology and Neurology (JC), Mayo Clinic, Rochester, Minnesota; and Clinical and Translational Science Institute (BL), University of Minnesota, Minneapolis, Minnesota
Abstract Background: To determine the normal variance of the mean macular ganglion cell layer (GCL) volume among subjects without significant ocular pathology using SPECTRALIS optical coherence tomography (OCT). Methods: Fifty subjects underwent a baseline scan using SPECTRALIS OCT followed by 2 more studies with (reg-ON) and without (reg-OFF) eye registration all taken at the same session. The mean GCL volume was measured using built-in SPECTRALIS software. Eyes with macular pathology were excluded. The reproducibility of the measurements of the GCL volume was evaluated with Bland-Altman plots and limits of agreement, intraclass correlation coefficient (ICC), and the coefficient of repeatability (CR). Results: A total of 98 eyes met criteria for the analysis. The mean GCL volume difference was 0.0002 ± 0.029 and -0.0005 ± 0.035 mm 3 for scans 1 versus 2 (baseline vs reg-ON) and 3 (baseline vs reg-OFF), respectively. The ICCs were 0.985 and 0.977 for the baseline vs reg-ON and reg-OFF groups. The CR for baseline vs reg-ON was 0.056 while CR for baseline vs reg-OFF was 0.069. Ninety percent of eyes fell within 0.04 mm 3 of test-retest reliability. Conclusions: Our model found a predictable threshold of 0.07 mm 3 or less for SPECTRALIS OCT mean GCL volume variance, which did not significantly change with eye registration in eyes without macular pathology. Clinicians may also consider a threshold of 0.04 mm 3 when determining stable vs progressive changes in mean GCL volume using this device.
Subject Retina; Retinal Ganglion Cells; Optical Coherence Tomography
OCR Text Show
Date 2022-09
Date Digital 2022-09
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Language eng
Format application/pdf
Type Text
Publication Type Journal Article
Source Journal of Neuro-Ophthalmology, September 2022, Volume 42, Issue 3
Collection Neuro-Ophthalmology Virtual Education Library: Journal of Neuro-Ophthalmology Archives: https://novel.utah.edu/jno/
Publisher Lippincott, Williams & Wilkins
Holding Institution Spencer S. Eccles Health Sciences Library, University of Utah
Rights Management © North American Neuro-Ophthalmology Society
ARK ark:/87278/s6x4m7pg
Setname ehsl_novel_jno
ID 2344188
Reference URL https://collections.lib.utah.edu/ark:/87278/s6x4m7pg
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