Characterization of Pactolus: transcription and function

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Title Characterization of Pactolus: transcription and function
Publication Type dissertation
School or College School of Medicine
Department Pathology
Author Margraf, Rebecca Lynn
Date 2003-05
Description Pactolus is a transmembrane protein found mainly on maturing to mature neutrophils and is homologous to ? integrin subunits. The majority of Pactolus is sequestered inside the cell until the neutrophil is activated or undergoes apoptosis. The gene structure of pactolus was determined in order to create a Pactolus deficient mouse. At the start of this thesis, Pactolus function was unknown, and the Pactolus deficient mouse proved to be instrumental in elucidating Pactolus function. Pactolus was not detected to have any intracellular signaling or in vivo functions, but in an in vitro assay mixing wild type or Pactolus deficient neutrophils with macrophages, Pactolus function was discovered. Pactolus appears to specifically interact with a macrophage receptor to facilitate apoptotic neutrophil phagocytosis by the macrophage and to downregulate macrophage TNF-? production. In a second project, to elucidate the tissue specific transcriptional control of the pactolus gene, the pactolus promoter was analyzed utilizing EMSA protocols. These data suggest a role for PU.1 and NF-k;B in pactolus gene regulation.
Type Text
Publisher University of Utah
Subject Mice; Neutrophil; Gene Regulation
Subject MESH Membrane Proteins; Neutrophils
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "The characterization of Pactolus: transcription and function". Spencer S. Eccles Health Sciences Library. Print version of "The characterization of Pactolus: transcription and function". available at J. Willard Marriott Library Special Collection. QP6.5 2003 .M37.
Rights Management © Rebecca Lynn Margraf.
Format application/pdf
Format Medium application/pdf
Format Extent 5,992,017 bytes
Identifier undthes,4491
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 5,992,048 bytes
ARK ark:/87278/s6cv4kkq
Setname ir_etd
ID 191494
Reference URL https://collections.lib.utah.edu/ark:/87278/s6cv4kkq
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