Studies on the heat labile hyaluronidase inhibitor of monkey serum

1. The nature of the rise of non-specific hyaluronidase in poliomyelitis infected monkeys was established. 2. Animals infected with the virus of poliomyelitis by means of the gastro-intestinal route failed to exhibit a rate in non-specific inhibition. Those animals infected by either the intranasal or intra-cerebral routes exhibited a rise in serum inhibitors levels at or near the onset of paralysis. 3. A possible relationship between virus antibody levels and the level of physiological inhibitor is suggested. 4. The kinetics of the enzymic hydrolysis of hyaluroic acid was studied in some detail by means of an improved turbidimetric method. The reaction order, enzyme-substrate dissociation constant, and the energy of activation were obtained by means of these studies. 5. The kinetics of the inhibi