Control of serine receptor signaling by the sensory adaptation system in escherichia coli

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Title Control of serine receptor signaling by the sensory adaptation system in escherichia coli
Publication Type dissertation
School or College College of Science
Department Biological Sciences
Author Han, Xuesheng
Date 2015-05
Description The chemotaxis signaling pathway of Escherichia coli is the best studied signal transduction mechanism in biology. Better understanding of this signal-processing machinery at the molecular level will foster new therapies for pathogenic infections and new designs of highly specific and sensitive biosensors. A sensory adaptation system plays a critical role in this chemotactic behavior. Sensory adaptation is regulated by covalent modifications of the chemoreceptors, mediated by CheR and CheB enzymes. This PhD research project explores the sensory adaptation mechanism of the serine receptor (Tsr) in E. coli. In this study, I showed that all adaptation sites of Tsr, including the fifth unorthodox site, worked in a similar way to regulate Tsr signal output. I also found that site 5 (Tsr-E502) and site 3 (Tsr-Q311) have differential signaling effects, mainly due to their different localizations on the methylation helices. Finally, I discovered unexpected signaling effects of CheR and CheB, the two adaptation enzymes. In summary, this thesis provides important insights into the sensory adaptation system and receptor input-output control in bacterial chemotaxis.
Type Text
Publisher University of Utah
Subject Adaptation; Chemoreceptor; Chemotaxis; Dynamic conformation; Methylation; Signal transduction
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Rights Management Copyright © Xuesheng Han 2015
Format application/pdf
Format Medium application/pdf
Format Extent 1,990,337 Bytes
Identifier etd3/id/3755
ARK ark:/87278/s6sf64hs
Setname ir_etd
ID 197306
Reference URL https://collections.lib.utah.edu/ark:/87278/s6sf64hs
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