Mechanical-tactile stimulation in a neonatal stress model alters depot-specific expression of proinflammatory cytokines, TNF-? and IL-6

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Title Mechanical-tactile stimulation in a neonatal stress model alters depot-specific expression of proinflammatory cytokines, TNF-? and IL-6
Publication Type thesis
School or College College of Health
Department Nutrition & Integrative Physiology
Author Warner, Grace
Date 2011-12
Description Premature infants are exposed to stressful events in the newborn intensive care unit (NICU). Neonatal stress is associated with increased adipose distribution to visceral depots (VAT). Adipose tissue, particularly VAT, produces two major cytokines associated with reducing insulin sensitivity, TNF-­?? and IL-­?6. Timed pregnant dams delivered at term (E21). Litters were culled to 10 pups (5 M, 5 F) and divided into three groups: control (CTL; maternal separation), neonatal stress (NS; maternal separation + injection + hypoxia/hyperoxia) and NS + MTS (10 min of stroking and limb movement). Treatments were given from D6-­?10 and tissue was harvested on D120. VAT and subcutaneous (SAT) depots measures were detected using MRI and DXA. Serum levels of insulin, TNF-­?? and IL-­?6 were measured with ELISA, and cytokine mRNA levels in VAT and SAT were measured by real-­?time PCR. Protein expression was determined through western blotting.
Type Text
Publisher University of Utah
Subject Mechanical-tactile stimulation; Neonatal stress; Premature infants; Proinflammatory cytokines
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Rights Management Copyright © Grace Warner 2011
Format application/pdf
Format Medium application/pdf
Format Extent 773,618 bytes
Identifier us-etd3,63552
Source Original housed in Marriott Library Special Collections, RJ25.5 2011 .W37
ARK ark:/87278/s6251zzc
Setname ir_etd
ID 194584
Reference URL https://collections.lib.utah.edu/ark:/87278/s6251zzc
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