Isolation and characterization of lymphocyte membrane antigen, AgF-1.

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Title Isolation and characterization of lymphocyte membrane antigen, AgF-1.
Publication Type dissertation
School or College School of Medicine
Department Pathology
Author Williams, Phillip Brock Carl.
Date 1976-03
Description A genetically defined, serologically identified antigen of the rat lymphocyte membrane (AgF-1) has been isolated. Viable spleen and lymph node cells, prepared by Ficoll-Hypaque density centrifugation from Fisher rats, were radioiodinated using soluble lactoperoxidase. Extracts, obtained with Nonidet P-40 were shown to contain numerous radio-labeled proteins, including cell-surface globulin. AgF-1 was isolated from these extracts by precipitation with a highly specific alloantibody in conjunction with xenospecific anti-globulin antibody and polyethylene glycol (PEG). The use of PEG greatly increased the efficiency of the double antibody technique. The putative antigenic peak was eluted from sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and specific antigenic activity was recovered. Removal of the SDS from there eluates was achieved by equilibration with urea and passage over an anion exchange resin. Renaturation, as evidenced by specific inhibition of complement-mediated cytotoxicity, occurred upon the removal of urea by dialysis. The molecular weight of the purified antigen was estimated to be 35-40,000 daltons by SDS-PAGE and was unaffected by reduction with 2-mercaptoethanol. Amino acid composition was roughly similar to those reported for the major histocompatibility antigens of the rat and other species.
Type Text
Publisher University of Utah
Subject Immunology; Biochemistry
Subject MESH Cell Membrane; Lymphocytes; Membrane Proteins
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Isolation and characterization of lymphocyte membrane antigen, AgF-1." Spencer S. Eccles Health Sciences Library. Print version of "Isolation and characterization of lymphocyte membrane antigen, AgF-1." available at J. Willard Marriott Library Special Collection. QR 6.5 1976 W55.
Rights Management © Phillip Brock Carl Williams.
Format application/pdf
Format Medium application/pdf
Identifier us-etd2,86
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship Grant from the National Institutes of Allergy and Infectious Diseases, AI-11956.
ARK ark:/87278/s6xp7kdv
Setname ir_etd
ID 192364
Reference URL https://collections.lib.utah.edu/ark:/87278/s6xp7kdv
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